We need long-term secure funding to provide you the information that you need at your fingertips. Protein interaction database and analysis system More Select item s and click on “Add to basket” to create your own collection here entries max. Transfectant cells were identified by immunoblot analysis, and five random clones with significantly increased PI31 levels were chosen for further analyses Fig. As an alternative approach to monitor PI31 effects on proteasomal proteolysis, we examined degradation of the ubiquitin Ub -R-GFP fusion protein, a relatively short-lived N-end-rule substrate 21 , in PI31 transfectant cells. Here, we demonstrate that, rather than inhibiting proteasome function, PI31 acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing. Comparative Toxicogenomics Database More
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Using proteomic analysis, An et al. UniGene gene-oriented nucleotide sequence clusters More Metabolic labeling of 20S complexes and 20S immunoprecipitation were performed as described These processing differences can lead to an enhanced or reduced cytotoxic T lymphocyte CTL recognition of infected or tumor cells 11— Available structures PDB Ortholog search: Protein interaction database and analysis system More But space agencies now el-pi31 multiple heavenly bodies in their sights, from Mars to Europa to Enceladus.
To explore the effects of this cell-derived proteasome inhibitor in intact cells, we transfected the mouse embryonic cell line Ad5E1 MEC 24which expresses the adenovirus type 5 early 1 E1 region and exhibits a low endogenous PI31 expression, with the coding sequences of mouse PI PI31 is a modulator of proteasome formation and antigen processing Dietmar M.
Taken together, these data demonstrate that in contrast to previously obtained in vitro evidence, PI31 does not function as a general inhibitor sll-pi31 proteasome activity in vivo.
Proteasome inhibitor PI31 subunit
Chromosome 20 human . Thus, PItransfectants show no apparent defects in degradation of ubiquitinylated proteins.
Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to Sl-pi13 damage. This page was last edited on 11 Septemberat This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.
ProteomicsDB human proteome resource More The displayed sequence is further processed into a mature form.
Mutation analysis revealed that the inhibitory function of PI31 resided within its proline-rich C-terminal domain. Reactome Other locations membrane Source: PI31 had no activity against other proteinases tested. Phosphoserine Combined sources Manual assertion inferred from combination of experimental and computational evidence i Ref.
Therefore, we suggest that PI31 plays a regulatory role by preventing the formation of immunoproteasomes sl-pi311 the absence of infection. Chordata Protein Annotation Program.
Belongs to the proteasome inhibitor PI31 family.
OMIM Entry – * – PROTEASOME INHIBITOR SUBUNIT 1; PSMF1
Hunting for microbial life throughout the solar system. PNAS October 29, 99 22 ; https: Edited by Charles A. Taken together, sl-p3i1 in agreement with other reports, these findings imply that, in cells with high MHC class I expression levels, MHC class I assembly is limited by peptide availability. One of these is PI31, which acts in vitro as an inhibitor of proteasome activity.
Also, levels of E1B protein synthesis are similar in parental and transfectant cells Fig. Protein Data Bank Europe More In contrast, cells treated with the synthetic proteasome inhibitor LLnL did accumulate multiubiquitin conjugates Fig.
PI31 is a modulator of proteasome formation and antigen processing | PNAS
As shown in Fig. PI31 blocked ATP-dependent in vitro assembly of 26S proteasome from 20S proteasome and PA subcomplexes, but it had no effect on in vitro activity of the intact 26S proteasome. September 12, This is version of the entry and version 2 of the sequence.